Desaspidin and CO2 Fixation by Spinach Chloroplasts
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چکیده
منابع مشابه
Desaspidin and CO(2) Fixation by Spinach Chloroplasts.
Baltscheffsky and de Kiewiet (1) proposed that desaspidin uncoupled photosynthetic phosphorylation in spinach chloroplasts. Arnon and coworkers (2, 3) have extended this work and on the basis of their finding that 1 ttm desaspidin inhibited cyclic phosphorylation completely while not affecting noncyclic phosphorylation, claim that desaspidin is a specific uncouipler at the site of cyclic phosph...
متن کاملCarbon dioxide fixation in the light and in the dark by isolated spinach chloroplasts.
Factors affecting CO(2) fixation in the spinach (Spinacia oleracea) chloroplast were investigated. Free magnesium ions are shown to be highly inhibitory for photosynthetic CO(2) fixation in isolated intact spinach chloroplasts. The pH optimum for CO(2) fixation is about 8.5 but is dependent upon the reaction medium. Conditions are defined under which chloroplasts illuminated in the absence of C...
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Digitonin has been used successfully to disrupt particulate structures such as mitochondria without loss of their capacity for causing oxidative phosphorylation (1, 2). The present report describes a procedure whereby spinach chloroplasts were extensively disrupted by digitonin extraction to give a preparation of small fragments which could cause an appreciable rate of photophosphorylation. In ...
متن کاملOxidative photosynthetic phosphorylation by spinach chloroplasts.
In an earlier note, evidence was presented for the occurrence of a new type of chloroplast-catalyzed photosynthetic phosphorylation called “oxidative photophosphorylation” (I). When spinach chloroplasts are illuminated in the presence of orthophosphate, adenosine diphosphate, and a dye such as trichlorophenol indophenol, adenosine triphosphate is generated provided oxygen is available, but not ...
متن کاملThe enhancement of CO2 fixation in isolated chloroplasts by low sulfite concentrations and by ascorbate.
The enhancement of CO2 fixation in isolated, intact spinach chloroplasts by ascorbate or by low sulfite concentrations (less than 1 mM) is strongly reduced or even abolished by the addition of superoxide dismutase (SOD). By the use of 35SO3(2-) it is demonstrated that the rate of sulfate formation is much lower than the sulfite induced increase in CO2 fixation. This indicates that the superoxid...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1966
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.41.7.1240